The contichromcube & HPLC continuous flow chromatography system, with the optimized 2-column design, can run a variety of chromatography modes, such as standard batch process, capture SMB, MCSGP (multi column countercurrent solvent gradient purification), N-rich, etc., which realizes the perfect combination of new chromatography technology and the design of software and hardware of the chromatography system.
System main interface
The main interface of the system adopts graphic design to display the information of pump speed and flow path, which is intuitive and easy to operate.
Contichrom Cube & HPLC function summary
Detailed function introduction
1) single column chromatography mode
The system is equipped with batch wizard software, which can run single column chromatography mode easy to design and operate.
Integration of two-step chromatography into one-step chromatography
All contichrom systems can integrate two chromatography steps (such as capture and purification) into a single process. The products of the first step can be diluted directly, so the second step can be carried out directly. Therefore, the intermediate intermittent process is omitted, which is very suitable for high-throughput purification.
Capture SMB
Due to the flow through effect, 40-60% of the fillers in the traditional chromatography process can not be fully utilized, so the load and treatment capacity are greatly limited. Capture SMB is used to optimize the capture process. As the simplest and most powerful 2-column design, it can significantly improve the purification efficiency and greatly reduce the amount of filler.
Capture SMB is to flow the sample of the first column through and continue to load the sample to the second column. After the first column is fully loaded, it can finish the sample washing, and continue to elute, regenerate and rebalance. At this time, the second column will independently load the sample. By switching the column position operation, the elution, regeneration, sample loading and other operations of the two chromatographic columns can be alternately connected to ensure continuous sample loading, so as to realize the perfect combination of process and system hardware design, maximize the filler utilization and greatly reduce the accumulated consumption.
For example: mabselect sure: static load 60 g / L, service life 200 cycles; handling capacity 600 kg mAb, packing volume 100 L.
4）MCSGP
In traditional chromatography, the trade-off between yield and purity is often needed. How to obtain high-purity products at the same time of ensuring high recovery rate has always been the difficulty of chromatography purification process.
MCSGP（Multicolumn Countercurrent Solvent Gradient Purification），The overlapped part can be recovered and the chromatographic operation of circulation can be carried out, so as to purify the product to the maximum extent, so that both purity and yield can be achieved.
Application case: the yield of kadcyla, a Roche ADC drug, was increased from 34% to 61% by MCSGP process, with an increase rate of 80%.
5）N-Rich
N-rich process is the enrichment process of trace substances, such as selectively enriching the impurities related to products in a cycle of chromatography process, to be enriched to the target amount, and then elution recovery. This method is especially suitable for the separation of impurities related to products, such as isomers and aggregates, and also for the discovery research.
Application case: the enrichment of synthetic peptide impurities, the enrichment multiple is as high as 600 times.
