Silica and other bonded fillers with polar functional groups, such as amino (NH2, APS) and cyanogroup (CN). Due to the strong polarity of the silicon hydroxyl group (SiOH) or other groups on the surface of the silica gel, the order of separation is based on the polarity of the components in the sample, that is, the components with weak polarity are first washed out of the column. Is chromatography with mobile phase polar stationary phase is relatively lower, such as: is ethane (Hexane), Chloroform (Chloroform), and Methylene Chloride (Methylene Chloride), etc.