Ion exchange chromatographic column is based on the difference of the ability of ion exchange between the separated components and the stationary phase. Ion exchange chromatography column of the stationary phase is commonly ion exchange resin, the resin molecular structure can be ionized in the active center, will need the ions in the separation of components with the active center of ion exchange, the formation of ion exchange equilibrium, thus in the liquid phase formed between the stationary phase, stationary phase and for separation of components of the inherent ions between competing ion exchange center of stationary phase, and with the movement of the mobile phase movement, finally realizes the separation.