Affinity chromatography column is the use of enzymes and substrates or inhibitors, antigen and antibody, hormones and receptors, exogenous lectin and polysaccharides and nucleic acid base pairs, the specific interaction between the material with the conclusion of the interaction between insoluble form covalent bonding compound supporter, used for chromatographic stationary phase, the other party from choose the reversible complex mixture to intercept, achieve the goal of purification.